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pET21a Ec-iPGM-bio
(Plasmid #162575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 7029
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E. coli iPGM bio-6His
  • Alt name
    Ec iPGM bio
  • Species
    E. coli
  • Insert Size (bp)
    1626
  • GenBank ID
    UniProtKB - P37689 (GPMI_ECOLI)
  • Promoter T7
  • Tags / Fusion Proteins
    • T7 tag (N terminal on backbone)
    • biotinylation sequence (C terminal on insert)
    • thrombin cleavage site (C terminal on insert)
    • 6His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See also van Neer et al. (2022) "Serum-Stable and Selective Backbone-N-Methylated Cyclic Peptides That Inhibit Prokaryotic Glycolytic Mutases" ACS Chem Biol. PMID 35904259; doi: https://doi.org/10.1021/acschembio.2c00403.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a Ec-iPGM-bio was a gift from James Inglese (Addgene plasmid # 162575 ; http://n2t.net/addgene:162575 ; RRID:Addgene_162575)

  • For your References section:

    Structure-activity relationship of ipglycermide binding to phosphoglycerate mutases. Wiedmann M, Dranchak PK, Aitha M, Queme B, Collmus CD, Kashipathy MM, Kanter L, Lamy L, Rogers JM, Tao D, Battaile KP, Rai G, Lovell S, Suga H, Inglese J. J Biol Chem. 2021 Apr 1:100628. doi: 10.1016/j.jbc.2021.100628. 10.1016/j.jbc.2021.100628 PubMed 33812994
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