Aurli_150841_GZG
(Plasmid #162563)

• Purpose: Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.
• Depositing Lab: Jackie Collier
• Publication: Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad029. doi: 10.1093/gbe/evad029.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 9190
• Modifications to backbone: Also added two 2 kb arms of homology from Aurli_150841 (one upstream and one downstream) of the zeocin construct (GZG).
• Vector type: Unspecified
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shble
• Alt name: BleoR
• Species: Streptoalloteichus hindustanus
• Insert Size (bp): 375
• GenBank ID: X52869.1 EF032505.1
• Promoter: GAPDH

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was constructed using Infusion Cloning (Clontech/ Takara)