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Addgene

pGEX-4T-3-SpSuc1
(Plasmid #162558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-3
  • Backbone size w/o insert (bp) 4968
  • Total vector size (bp) 5310
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Schizosaccharomyces pombe Suc1
  • Alt name
    p13
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    342
  • Entrez Gene
    suc1 (a.k.a. SPBC1734.14c)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AGCGGATAACAATTTCACACAGG
  • 3′ sequencing primer CCGGGAGCTGCATGTGTCAGAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-3-SpSuc1 was a gift from Iain Hagan (Addgene plasmid # 162558 ; http://n2t.net/addgene:162558 ; RRID:Addgene_162558)

  • For your References section:

    Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition. Singh P, Halova L, Hagan IM. Methods Mol Biol. 2021;2329:123-142. doi: 10.1007/978-1-0716-1538-6_10. 10.1007/978-1-0716-1538-6_10 PubMed 34085220
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