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Addgene

Cip-pAct1-Mnase
(Plasmid #162556)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162556 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Cip-Act1-Cyc
  • Backbone size w/o insert (bp) 6305
  • Total vector size (bp) 6866
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3FLAG-Mnase-SV40
  • Species
    Synthetic
  • Insert Size (bp)
    561
  • Promoter Actine

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer AGGGTTTTCCCAGTCACG
  • 3′ sequencing primer GAGCGGATAACAATTTCACAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cip-pAct1-Mnase was a gift from Adnane Sellam (Addgene plasmid # 162556 ; http://n2t.net/addgene:162556 ; RRID:Addgene_162556)

  • For your References section:

    High-Resolution Genome-Wide Occupancy in Candida spp. Using ChEC-seq. Tebbji F, Khemiri I, Sellam A. mSphere. 2020 Oct 14;5(5). pii: 5/5/e00646-20. doi: 10.1128/mSphere.00646-20. 10.1128/mSphere.00646-20 PubMed 33055256
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