pcDNA 3.1(-) VSFP2.1
(Plasmid #16255)

• Depositing Lab: Thomas Knopfel
• Publication: Dimitrov et al PLoS ONE. 2007 . 2(5):e440.

Backbone

• Vector backbone: pcDNA3.1 (-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5317
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VSFP2.1
• Alt name: Voltage-Sensitive Fluorescent Protein 2.1
• Species: Ciona intestinalis
• Insert Size (bp): 2253

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cDNA coding sequence for Ci-VSP was a gift from Dr. Okamura (NIPS, Okasaki, Japan). The cDNA coding sequence for Cerulean was a gift from Dr. Piston (Vanderbilt University, USA). The cDNA coding sequence for Citrine was a gift from Dr. Griesbeck (MPI Martinsried, Germany).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Voltage sensor domain of Ci-VSP fused to a pair of fluorescent proteins consisting of a C-terminally truncated CFP (Cerulean) and YFP (Citrine)