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pcDNA3 myc card9 mutant
(Plasmid #16254)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    card9 mutant
  • Alt name
    Card 9
  • Alt name
    caspase recruitment domain family, member 9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    S528A, T531A, T533A, S536A
  • GenBank ID
    NM_052813
  • Entrez Gene
    CARD9 (a.k.a. CANDF2, IMD103, hCARD9)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI / BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Identical to pcDNA3-myc-card9, except contains S528A, T531A, T533A, S536A mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 myc card9 mutant was a gift from William Kaelin (Addgene plasmid # 16254 ; http://n2t.net/addgene:16254 ; RRID:Addgene_16254)

  • For your References section:

    pVHL Acts as an adaptor to promote the inhibitory phosphorylation of the NF-kappaB agonist Card9 by CK2. Yang H, Minamishima YA, Yan Q, Schlisio S, Ebert BL, Zhang X, Zhang L, Kim WY, Olumi AF, Kaelin WG. Mol Cell. 2007 Oct 12. 28(1):15-27. 10.1016/j.molcel.2007.09.010 PubMed 17936701
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