pcDNA3 myc card9
(Plasmid #16253)

• Depositing Lab: William Kaelin
• Publication: Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: card9
• Alt name: Card 9
• Alt name: caspase recruitment domain family, member 9
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: NM_052813
• Entrez Gene: CARD9 (a.k.a. CANDF2, IMD103, hCARD9)
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI / BglII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Card9 cDNA from Origene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A full-length Card9 cDNA clone (NM_052813) was purchased from Origene. The open reading frame was PCR amplified with primers that introduced a 5′BamHI site and 3′EcoRI site to facilitate subcloning into a pcDNA3 plasmid (Invitrogen) that introduced an N-terminal Myc-epitope tag.