IR25a-T2A-QF2
(Plasmid
#162522)
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PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepuc19
- Backbone size w/o insert (bp) 2680
- Total vector size (bp) 8639
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIr25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
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SpeciesSynthetic; Aedes aegypti
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Insert Size (bp)5988
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IR25a-T2A-QF2 was a gift from Leslie Vosshall (Addgene plasmid # 162522 ; http://n2t.net/addgene:162522 ; RRID:Addgene_162522) -
For your References section:
Non-canonical odor coding in the mosquito. Herre M, Goldman OV, Lu TC, Caballero-Vidal G, Qi Y, Gilbert ZN, Gong Z, Morita T, Rahiel S, Ghaninia M, Ignell R, Matthews BJ, Li H, Vosshall LB, Younger MA. Cell. 2022 Aug 18;185(17):3104-3123.e28. doi: 10.1016/j.cell.2022.07.024. 10.1016/j.cell.2022.07.024 PubMed 35985288
Map uploaded by the depositor.
