GFP-GLT1b-V5
(Plasmid
#162518)
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PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1 (Clontech)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGLT1b
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SpeciesR. norvegicus (rat)
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Entrez GeneSlc1a2 (a.k.a. Eaat2, Glt, Glt-1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-GLT1b-V5 was a gift from Josef Kittler (Addgene plasmid # 162518 ; http://n2t.net/addgene:162518 ; RRID:Addgene_162518) -
For your References section:
Neuronal activity mediated regulation of glutamate transporter GLT-1 surface diffusion in rat astrocytes in dissociated and slice cultures. Al Awabdh S, Gupta-Agarwal S, Sheehan DF, Muir J, Norkett R, Twelvetrees AE, Griffin LD, Kittler JT. Glia. 2016 Jul;64(7):1252-64. doi: 10.1002/glia.22997. 10.1002/glia.22997 PubMed 27189737