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Addgene

GFP-GLT1a-V5
(Plasmid #162517)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1 (Clontech)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLT1a
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Slc1a2 (a.k.a. Eaat2, Glt, Glt-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-GLT1a-V5 was a gift from Josef Kittler (Addgene plasmid # 162517 ; http://n2t.net/addgene:162517 ; RRID:Addgene_162517)

  • For your References section:

    Neuronal activity mediated regulation of glutamate transporter GLT-1 surface diffusion in rat astrocytes in dissociated and slice cultures. Al Awabdh S, Gupta-Agarwal S, Sheehan DF, Muir J, Norkett R, Twelvetrees AE, Griffin LD, Kittler JT. Glia. 2016 Jul;64(7):1252-64. doi: 10.1002/glia.22997. 10.1002/glia.22997 PubMed 27189737
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