AAV-mPGK-eGFP (beta-globin intron)
(Plasmid #162513)

• Purpose: AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron
• Depositing Lab: Bart Nieuwenhuis
• Publication: Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41434-020-0169-1. doi: 10.1038/s41434-020-0169-1.

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Backbone

• Vector backbone: Addgene plasmid #24593
• Backbone manufacturer: Patrick Aebischer
• Total vector size (bp): 5271
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 720
• Promoter: mPGK

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: Not applicable
• 3′ sequencing primer: Not applicable

Resource Information

• Supplemental Documents:
  • AAV-mPGK-eGFP (beta-globin intron).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry