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AAV-hSYN-eGFP
(Plasmid #162512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRUF20B-SEW
  • Backbone manufacturer
    Deniz Kirik
  • Total vector size (bp) 4640
  • Modifications to backbone
    AAV-hSYN-eGFP was made by removing the WPRE from plasmid pTRUF20B-SEW by HincII digestion
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    720
  • Promoter hSYN

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-hSYN-eGFP was a gift from Bart Nieuwenhuis (Addgene plasmid # 162512 ; http://n2t.net/addgene:162512 ; RRID:Addgene_162512)

  • For your References section:

    Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters. Nieuwenhuis B, Haenzi B, Hilton S, Carnicer-Lombarte A, Hobo B, Verhaagen J, Fawcett JW. Gene Ther. 2020 Jun 23. pii: 10.1038/s41434-020-0169-1. doi: 10.1038/s41434-020-0169-1. 10.1038/s41434-020-0169-1 PubMed 32576975
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