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Addgene

EFS-ABE7.10-P2A-GFP
(Plasmid #162510)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Modifications to backbone
    replace CMV prmoter to EFS promoter
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE7.10-P2A-GFP
  • Species
    Synthetic
  • Promoter EFS
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    from pCMV-ABE7.10 (Addgene plasmid #102919)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EFS-ABE7.10-P2A-GFP was a gift from Yongsub Kim (Addgene plasmid # 162510 ; http://n2t.net/addgene:162510 ; RRID:Addgene_162510)

  • For your References section:

    Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing. Shin HR, See JE, Kweon J, Kim HS, Sung GJ, Park S, Jang AH, Jang G, Choi KC, Kim I, Kim JS, Kim Y. Nucleic Acids Res. 2021 Feb 26;49(4):2390-2399. doi: 10.1093/nar/gkab052. 10.1093/nar/gkab052 PubMed 33544854
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