pCMV-ABE7.10-P2A-GFP
(Plasmid
#162509)
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PurposeExpress ABE7.10-P2A-GFP by CMV promoter in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162509 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameABE7.10-P2A-GFP
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SpeciesSynthetic
- Promoter CMV
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Tag
/ Fusion Protein
- NLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byfrom pCMV-ABE7.10 (Addgene plasmid #102919)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-ABE7.10-P2A-GFP was a gift from Yongsub Kim (Addgene plasmid # 162509 ; http://n2t.net/addgene:162509 ; RRID:Addgene_162509) -
For your References section:
Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing. Shin HR, See JE, Kweon J, Kim HS, Sung GJ, Park S, Jang AH, Jang G, Choi KC, Kim I, Kim JS, Kim Y. Nucleic Acids Res. 2021 Feb 26;49(4):2390-2399. doi: 10.1093/nar/gkab052. 10.1093/nar/gkab052 PubMed 33544854
