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Addgene

SBP-GFP-Tac-TC
(Plasmid #162506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5114
  • Total vector size (bp) 6059
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP and SBP tagged partial length Tac (TMD and cytosolic tail)
  • Alt name
    IL2 receptor alpha subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    945
  • GenBank ID
    NM_000417.3
  • Entrez Gene
    IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
  • Tags / Fusion Proteins
    • SBP (N terminal on insert)
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-CF
  • 3′ sequencing primer SV40-PArev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SBP-GFP-Tac-TC was a gift from Lei Lu (Addgene plasmid # 162506 ; http://n2t.net/addgene:162506 ; RRID:Addgene_162506)

  • For your References section:

    Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314
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