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Addgene

ST-2xTstem(5A)-GFP
(Plasmid #162504)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162504 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmoxGFP-N1
  • Backbone manufacturer
    A gift from E. Snapp (Addgene plasmid # 68070)
  • Backbone size w/o insert (bp) 3959
  • Total vector size (bp) 6086
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP tagged ST6Gal1 mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2127
  • Mutation
    One Tac stem region is inserted within ST6Gal1 and the other Tac stem region with mutations is inserted between ST6Gal1 and GFP.
  • GenBank ID
    BC040009.1 NM_000417.3
  • Entrez Gene
    ST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
  • Entrez Gene
    IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40-PArev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ST-2xTstem(5A)-GFP was a gift from Lei Lu (Addgene plasmid # 162504 ; http://n2t.net/addgene:162504 ; RRID:Addgene_162504)

  • For your References section:

    Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314
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