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Addgene

ST-Tstem(5A)-GFP
(Plasmid #162502)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162502 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmoxGFP-N1
  • Backbone manufacturer
    A gift from E. Snapp (Addgene plasmid # 68070)
  • Backbone size w/o insert (bp) 4697
  • Total vector size (bp) 6005
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1311
  • Mutation
    The mutated Tac stem region is inserted within ST6Gal1
  • GenBank ID
    BC040009.1 NM_000417.3
  • Entrez Gene
    ST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
  • Entrez Gene
    IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ST-Tstem(5A)-GFP was a gift from Lei Lu (Addgene plasmid # 162502 ; http://n2t.net/addgene:162502 ; RRID:Addgene_162502)

  • For your References section:

    Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314
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