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Addgene

pSV2-c-erbB2
(Plasmid #16250)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV2
  • Backbone size w/o insert (bp) 4200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-erbB2
  • Alt name
    Her2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • Entrez Gene
    ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tadashi Yamamoto, Univ of Tokyo
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV2-c-erbB2 was a gift from Mien-Chie Hung (Addgene plasmid # 16250 ; http://n2t.net/addgene:16250 ; RRID:Addgene_16250)

  • For your References section:

    A novel splice variant of HER2 with increased transformation activity. Kwong KY, Hung MC. Mol Carcinog. 1998 Oct . 23(2):62-8. 10.1002/(SICI)1098-2744(199810)23:2<62::AID-MC2>3.0.CO;2-O PubMed 9808159
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