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Addgene

GFP-Cstem-Tac(5A)
(Plasmid #162497)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162497 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4770
  • Total vector size (bp) 5655
  • Modifications to backbone
    The signal peptide of Tac was inserted before GFP tag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD8a stem region and Tac full length with mutated stem region
  • Alt name
    IL2 receptor alpha subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    885
  • Mutation
    The five threonine residues are mutated into alanine residues in the stem region of Tac
  • GenBank ID
    NM_000417.3 NM_001145873.1
  • Entrez Gene
    IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
  • Entrez Gene
    CD8A (a.k.a. CD8, CD8alpha, Leu2, p32)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-CF
  • 3′ sequencing primer SV40-PArev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Cstem-Tac(5A) was a gift from Lei Lu (Addgene plasmid # 162497 ; http://n2t.net/addgene:162497 ; RRID:Addgene_162497)

  • For your References section:

    Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314
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