GFP-Tac-TC
(Plasmid #162492)

• Purpose: Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cells
• Depositing Lab: Lei Lu
• Publication: Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4770
• Total vector size (bp): 4866
• Modifications to backbone: The signal peptide of Tac was inserted before GFP tag
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: partial length Tac (TMD and cytosolic tail)
• Alt name: IL2 receptor alpha subunit
• Species: H. sapiens (human)
• Insert Size (bp): 96
• Mutation: Tac is in partial length with its TMD and cysotolic tail
• GenBank ID: NM_000417.3
• Entrez Gene: IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: EGFP-CF
• 3′ sequencing primer: SV40-PArev

Resource Information

• Supplemental Documents:
  • GFP-Tac-TC.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry