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Addgene

pNG1304
(Plasmid #162488)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis6-HelD
  • Backbone manufacturer
    https://doi.org/10.1093/nar/gku113
  • Modifications to backbone
    Introduced a K239A point mutation in helD gene
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HelD
  • Species
    Bacillus subtillis 168
  • Insert Size (bp)
    2424
  • Mutation
    Changed Lysine 239 to Alanine (K239A)
  • GenBank ID
    CAB15350.1
  • Entrez Gene
    helD (a.k.a. BSU_33450, BSU33450)
  • Tag / Fusion Protein
    • 6His-V5 (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNG1304 was a gift from Peter Lewis (Addgene plasmid # 162488 ; http://n2t.net/addgene:162488 ; RRID:Addgene_162488)

  • For your References section:

    Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD. Newing TP, Oakley AJ, Miller M, Dawson CJ, Brown SHJ, Bouwer JC, Tolun G, Lewis PJ. Nat Commun. 2020 Dec 18;11(1):6420. doi: 10.1038/s41467-020-20157-5. 10.1038/s41467-020-20157-5 PubMed 33339820
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