pPBbsr-6080NES(Epac-sensor)
(Plasmid #162487)

• Purpose: hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
• Depositing Lab: Michiyuki Matsuda
• Publication: Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.

Backbone

• Vector backbone: pPB
• Backbone size w/o insert (bp): 6800
• Total vector size (bp): 11368
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Epac-sensor
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 4500
• Entrez Gene: RAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
• Promoter: CAG
• Tag / Fusion Protein:
  • YPet and Turquoise2GL

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pCAGGS-5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry