pT2ADW-4146NES(ROCK)
(Plasmid #162485)

• Purpose: For generating transgenic mice expressing ROCK biosensor based on FRET.
• Depositing Lab: Michiyuki Matsuda
• Publication: Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-9440(20)30459-4. doi: 10.1016/j.ajpath.2020.09.012.

Backbone

• Vector backbone: pT2ADW
• Backbone size w/o insert (bp): 7300
• Modifications to backbone: pT2ADW was constructed as follows: The D4Z4 insulator was inserted before the CAG promoter of pT2AL200R175 carrying Tol2 recombination sites. Then, the WPRE sequence of pCSII-EF was inserted before the poly A sequence.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ROCK biosensor based on FRET.
• Alt name: pT2ADW-ROCK
• Species: Synthetic
• Insert Size (bp): 2300
• Promoter: CAG
• Tag / Fusion Protein:
  • YPet and ECFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pCAGGS-5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry