HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw
(Plasmid
#162477)
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PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBPGUw
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Vector typeCre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCreC
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw was a gift from Benjamin White (Addgene plasmid # 162477 ; http://n2t.net/addgene:162477 ; RRID:Addgene_162477) -
For your References section:
Cre-assisted fine-mapping of neural circuits using orthogonal split inteins. Luan H, Kuzin A, Odenwald WF, White BH. Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041. 10.7554/eLife.53041 PubMed 32286225