pNeuLite
(Plasmid #16247)

• Depositing Lab: Mien-Chie Hung
• Publication: Xing et al Nat Med. 2000 Feb . 6(2):189-95.

Backbone

• Vector backbone: pGL2-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Her-2/neu promoter
• Alt name: Her2
• Species: H. sapiens (human)
• Entrez Gene: ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The promoter region of human HER-2/neu was amplified with a pair of primers (KO11: 5'−GATAGGATCC GGGGGTCCTG GAAGCC−3' and KO2: 5'−GGGCAGATCT GGTTTTCCGG TCCCAATGGA−3'). Also named pNeuLit.