pJYDC3
(Plasmid
#162460)
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Purpose(Empty Backbone) Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162460 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNew backbone pJYD
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Backbone manufacturerDr. Jiri Zahradnik
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Vector typeShuttle vector bacteria/yeast
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Selectable markersTRP1
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Tags
/ Fusion Proteins
- DnbALFA designed ALFA nanobody (N terminal on backbone)
- HA tag (N terminal on backbone)
- HDEL sequence (C terminal on backbone)
- Myc tag (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.12.16.423176v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJYDC3 was a gift from Gideon Schreiber (Addgene plasmid # 162460 ; http://n2t.net/addgene:162460 ; RRID:Addgene_162460) -
For your References section:
An enhanced yeast display platform demonstrates the binding plasticity under various selection pressures. Zahradník J, Dey D, Marciano S, Schreiber G. bioRxiv Preprint 2020 10.1101/2020.12.16.423176
Map uploaded by the depositor.
