pJYDN2
(Plasmid
#162454)
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Purpose(Empty Backbone) Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.
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Depositing Lab
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Publication
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Sequence Information
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Sequences (3) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNew backbone pJYD
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Backbone manufacturerDr. Jiri Zahradnik
- Backbone size (bp) 5160
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Vector typeShuttle vector bacteria/yeast
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Selectable markersTRP1
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Tags
/ Fusion Proteins
- Ha and myc (C terminal on backbone)
- DnbALFA nanobody (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.12.16.423176v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJYDN2 was a gift from Gideon Schreiber (Addgene plasmid # 162454 ; http://n2t.net/addgene:162454 ; RRID:Addgene_162454) -
For your References section:
An enhanced yeast display platform demonstrates the binding plasticity under various selection pressures. Zahradník J, Dey D, Marciano S, Schreiber G. bioRxiv Preprint 2020 10.1101/2020.12.16.423176
Map uploaded by the depositor.
