CCR5-SZ190b-sfGFP
(Plasmid #162447)

• Purpose: Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration
• Depositing Lab: Shuguang Zhang
• Publication: Qing et al iScience. 2020 Oct 28;23(12):101670. doi: 10.1016/j.isci.2020.101670. eCollection 2020 Dec 18.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 6100
• Total vector size (bp): 6665
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C-C chemokine receptor type 5
• Alt name: CD195
• Species: H. sapiens (human)
• Mutation: truncation from aa88 to aa249
• Entrez Gene: CCR5 (a.k.a. CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • CCR5-SZ190b-sfGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry