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Addgene

HA-Akt CA (myr-HA-Akt)
(Plasmid #16244)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16244 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Akt1
  • Species
    M. musculus (mouse)
  • Mutation
    Constitutively active due to myristoylation sequence (MGSSKSKPK).
  • Entrez Gene
    Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
  • Tags / Fusion Proteins
    • Myr (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Philip Tsichlis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also see references:

Cancer Res. 2000 Dec 15;60(24):6841-5.

Clin Cancer Res. 2004 Jun 1;10(11):3815-24.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Akt CA (myr-HA-Akt) was a gift from Mien-Chie Hung (Addgene plasmid # 16244)

  • For your References section:

    HER-2/neu blocks tumor necrosis factor-induced apoptosis via the Akt/NF-kappaB pathway. Zhou BP, Hu MC, Miller SA, Yu Z, Xia W, Lin SY, Hung MC. J Biol Chem. 2000 Mar 17. 275(11):8027-31. 10.1074/jbc.275.11.8027 PubMed 10713122
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