pGEX-4T MDM2 S186D
(Plasmid
#16239)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-3
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Backbone manufacturerPharmacia
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMDM2
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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MutationSer186 replaced by Asp
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Entrez GeneMDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T MDM2 S186D was a gift from Mien-Chie Hung (Addgene plasmid # 16239 ; http://n2t.net/addgene:16239 ; RRID:Addgene_16239) -
For your References section:
HER-2/neu induces p53 ubiquitination via Akt-mediated MDM2 phosphorylation. Zhou BP, Liao Y, Xia W, Zou Y, Spohn B, Hung MC. Nat Cell Biol. 2001 Nov . 3(11):973-82. 10.1038/ncb1101-973 PubMed 11715018