pcDNA3 MDM2 S166D S186D
(Plasmid #16236)

• Depositing Lab: Mien-Chie Hung
• Publication: Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MDM2
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: Ser166 and Ser186 replaced by Asp
• Entrez Gene: MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry