pCA1.332 pDUOTK1-L1
(Plasmid
#162351)
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PurposeLevel 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAT.000
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Modifications to backboneAmpicillin resistance cassette has been removed
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameeYFP
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Insert Size (bp)720
Gene/Insert 2
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Gene/Insert nameLacZ alpha
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Insert Size (bp)537
Gene/Insert 3
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Gene/Insert namemTagBFP
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Insert Size (bp)702
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCA1.332 pDUOTK1-L1 was a gift from Alistair McCormick (Addgene plasmid # 162351 ; http://n2t.net/addgene:162351 ; RRID:Addgene_162351) -
For your References section:
Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species. Gale GAR, Wang B, McCormick AJ. Front Microbiol. 2021 Jan 15;11:624011. doi: 10.3389/fmicb.2020.624011. eCollection 2020. 10.3389/fmicb.2020.624011 PubMed 33519785