pTet-O-RUNX3-T2A-PuroR
(Plasmid #162349)

• Purpose: Lentiviral expression of RUNX3 under the control of the TetON promoter
• Depositing Lab: Charles Gersbach
• Publication: Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460.

Backbone

• Vector backbone: Tet-O-FUW
• Backbone size w/o insert (bp): 8000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RUNX3
• Species: H. sapiens (human)
• Insert Size (bp): 1245
• Entrez Gene: RUNX3 (a.k.a. AML2, CBFA3, PEBP2aC)
• Promoter: Tet-ON
• Tag / Fusion Protein:
  • T2A-PuroR (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pTet-O-RUNX3-T2A-PuroR.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was modifed from a gift from Marius Wernig (Addgene plasmid # 52047)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry