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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP
(Plasmid #162336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. pyogenes sgRNA
  • gRNA/shRNA sequence
    CTCGAGAGAGCAAACAGAG
  • Species
    S. pyogenes
  • Promoter hU6; mU6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP was a gift from Charles Gersbach (Addgene plasmid # 162336 ; http://n2t.net/addgene:162336 ; RRID:Addgene_162336)

  • For your References section:

    Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA. Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460. 10.1016/j.celrep.2020.108460 PubMed 33264623
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