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Addgene

pT-43
(Plasmid #16232)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16232 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30 (QIAGEN)
  • Backbone size w/o insert (bp) 3462
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    M15[pREP4]
  • Growth instructions
    Needs strain with lacIq
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SMT3
  • Alt name
    Yeast SUMO mature form (SMT3gg)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    294
  • Entrez Gene
    SMT3 (a.k.a. YDR510W)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT-43 was a gift from Yoshiko Kikuchi (Addgene plasmid # 16232 ; http://n2t.net/addgene:16232 ; RRID:Addgene_16232)

  • For your References section:

    Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro. Takahashi Y, Toh-E A, Kikuchi Y. J Biochem (Tokyo). 2003 Apr . 133(4):415-22. 10.1093/jb/mvg054 PubMed 12761287
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