pIR-CMV-SCN1A-Variant-1-IRES-mScarlet
(Plasmid #162278)

• Purpose: Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
• Depositing Lab: Al George
• Publication: DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. doi: 10.1016/j.jbc.2021.100298. Epub 2021 Jan 15.

Backbone

• Vector backbone: pIR-CMV-IRES-mScarlet
• Backbone size w/o insert (bp): 6053
• Total vector size (bp): 12216
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SCN1A Variant 1, stabilized
• Alt name: Nav1.1
• Species: H. sapiens (human)
• Insert Size (bp): 6163
• Mutation: IVS intron inserted after bp2946. Silent codon changes at c.879G>A, c.T897T>C, c.900T>C, c.903T>C and 4288 T>C.
• GenBank ID: NM_001165963
• Entrez Gene: SCN1A (a.k.a. DEE6, DEE6A, DEE6B, DRVT, EIEE6, FEB3, FEB3A, FHM3, GEFSP2, HBSCI, NAC1, Nav1.1, SCN1, SMEI)
• Promoter: CMV
• Tag / Fusion Protein:
  • IRES-mScarlet (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: PvuI (not destroyed)
• 5′ sequencing primer: V1-5
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pIR-CMV-SCN1A-Variant-1-IRES-mScarlet.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The SCN1A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions. Please visit https://www.biorxiv.org/content/10.1101/2020.11.05.370429v1 for bioRxiv preprint.