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pGEX6P1-mCherry-Nanobody (LaM-2)
(Plasmid #162276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    GE healthcare
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5377
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-Nanobody (LaM-2)
  • Alt name
    LaM-2
  • Species
    Lama glama
  • Insert Size (bp)
    396
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CGTATTGAAGCTATCCCAC
  • 3′ sequencing primer CGGCATCCGCTTACAGAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCherry-Nanobody (LaM-2) was originally developed by Dr. Rout et al. (see citation in the comments section)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional citation: Fridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyö D, Chait BT & Rout MP (2014) A robust pipeline for rapid production of versatile nanobody repertoires. Nat Meth 11: 1253–1260

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P1-mCherry-Nanobody (LaM-2) was a gift from Kazuhisa Nakayama (Addgene plasmid # 162276 ; http://n2t.net/addgene:162276 ; RRID:Addgene_162276)

  • For your References section:

    Molecular basis of ciliary defects caused by compound heterozygous IFT144/WDR19 mutations found in cranioectodermal dysplasia. Ishida Y, Kobayashi T, Chiba S, Katoh Y, Nakayama K. Hum Mol Genet. 2021 Apr 26;30(3-4):213-225. doi: 10.1093/hmg/ddab034. 10.1093/hmg/ddab034 PubMed 33517396
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