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Addgene

HTT delta-exon1
(Plasmid #162274)

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Item Catalog # Description Quantity Price (USD)
Plasmid 162274 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBacMam2-DiEx-LIC
  • Backbone manufacturer
    Oxford SGC
  • Vector type
    Mammalian Expression ; Baculovirus Expresssion

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HTT
  • Alt name
    HTT delta-exon1 construct, SGC Toronto
  • Alt name
    huntingtin:TOC017-E07:C242692
  • Species
    H. sapiens (human)
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Promoter CMV and P10
  • Tag / Fusion Protein
    • 10xHis-Flag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBM-F: caaaatgtcgtaacaactccgc
  • 3′ sequencing primer pFBM-R: tagttaagaataccagtcaatctttcac
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-terminal tag: M. C-terminal tag: aenlyfqshhhhhhhhhhdykddddk. SGC Clone ID: huntingtin:TOC017-E07:C242692.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HTT delta-exon1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 162274 ; http://n2t.net/addgene:162274 ; RRID:Addgene_162274)
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