6VAH
(Plasmid
#162273)
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PurposeBacterial Expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162273 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepNIC-CH
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Backbone manufacturerSGC (Addgene Plasmid ##26117)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTEAD2
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Alt nameTEAD2:PBC019-D11:C244026
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SpeciesH. sapiens (human)
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Insert Size (bp)693
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Entrez GeneTEAD2 (a.k.a. ETF, TEAD-2, TEF-4, TEF4)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N-terminal tag AA:M. C-terminal tag AA:VEHHHHHH. SGC Clone ID:TEAD2:PBC019-D11:C244026. SGC Clone ID:http://www.thesgc.org/structures/6VAH/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6VAH was a gift from Cheryl Arrowsmith (Addgene plasmid # 162273 ; http://n2t.net/addgene:162273 ; RRID:Addgene_162273)
