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Addgene

6XKC
(Plasmid #162271)

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Item Catalog # Description Quantity Price (USD)
Plasmid 162271 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28GST-LIC
  • Backbone manufacturer
    SGC (Addgene Plasmid #26101)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FEM1C
  • Alt name
    FEM1C:JMC130-A08:C242836
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    732
  • Entrez Gene
    FEM1C (a.k.a. EUROIMAGE686608, EUROIMAGE783647, FEM1A)
  • Promoter GST-F:tgaagctatcccacaaattg
  • Tag / Fusion Protein
    • 6xHis, GST and Thrombin (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-terminal tag AA:MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSSMGSSHHHHHHSSGLVPRGS. SGC Clone ID:FEM1C:JMC130-A08:C242836. SGC Clone ID:http://www.thesgc.org/structures/6XKC/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6XKC was a gift from Cheryl Arrowsmith (Addgene plasmid # 162271 ; http://n2t.net/addgene:162271 ; RRID:Addgene_162271)
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