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Addgene

6WAJ
(Plasmid #162267)

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Item Catalog # Description Quantity Price (USD)
Plasmid 162267 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid # 26096)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NLE1
  • Alt name
    NLE1:PBC021-B01:C244625
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    291
  • Entrez Gene
    NLE1 (a.k.a. Nle, FLJ10458)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-terminal tag AA:MHHHHHHSSGRENLYFQG. SGC Clone ID:NLE1:PBC021-B01:C244625. SGC Clone ID:http://www.thesgc.org/structures/6WAJ/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6WAJ was a gift from Cheryl Arrowsmith (Addgene plasmid # 162267 ; http://n2t.net/addgene:162267 ; RRID:Addgene_162267)
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