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Addgene

6PDM
(Plasmid #162264)

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Item Catalog # Description Quantity Price (USD)
Plasmid 162264 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #62304)
  • Vector type
    Baculovirus Expresssion

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRMT9
  • Alt name
    PRMT9:PBC015-H11:C242170, 9AY9
  • Alt name
    PDB ID: 6PDM, 7RBQ
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2157
  • Entrez Gene
    PRMT9 (a.k.a. PRMT10)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-terminal tag AA:MHHHHHHSSGRENLYFQG.
SGC Clone ID:PRMT9:PBC015-H11:C242170.
PDB:
https://www.thesgc.org/structures/6PDM/
https://www.thesgc.org/structures/7RBQ/
https://www.rcsb.org/structure/9AY9/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6PDM was a gift from Cheryl Arrowsmith (Addgene plasmid # 162264 ; http://n2t.net/addgene:162264 ; RRID:Addgene_162264)
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