6P0Q
(Plasmid
#162262)
-
PurposeBaculovirus expression for structure determination
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Log in to view industry pricing.
Backbone
-
Vector backbonepNIC-CTHO
-
Backbone manufacturerOxford SGC
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameWDR12
-
Alt nameWDR12:PBC016-D02:C242831
-
SpeciesH. sapiens (human)
-
Insert Size (bp)261
-
Entrez GeneWDR12 (a.k.a. YTM1)
- Promoter T7
-
Tag
/ Fusion Protein
- TEV-6xHis (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N-terminal tag AA:M. C-terminal tag AA:AENLYFQSHHHHHH. SGC Clone ID:WDR12:PBC016-D02:C242831. SGC Clone ID:http://www.thesgc.org/structures/6P0Q/
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
6P0Q was a gift from Cheryl Arrowsmith (Addgene plasmid # 162262 ; http://n2t.net/addgene:162262 ; RRID:Addgene_162262)
