Human Epigenetic Knockout Library
(Pooled Library #162256)
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Purpose
This lentiviral CRISPR knockout library targets human epigenetic genes.
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Vector Backbone
LentiCRISPRv2-Opti (Plasmid #163126) - expresses Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 162256 | Human Epigenetic KO Library | 1 | $430 | Add to Cart | |
Available to Academic and Nonprofits Only
Library Details
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SpeciesHuman
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Genes targeted2,508
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gRNAs20,051
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Controls50 intergenic region targeting gRNAs
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼20 µL
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Concentration25 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
How to cite this pooled library
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These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human Epigenetic Knockout Library was a gift from Kivanc Birsoy (Addgene #162256) -
For your References section:
ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase. Williams RT, Guarecuco R, Gates LA, Barrows D, Passarelli MC, Carey B, Baudrier L, Jeewajee S, La K, Prizer B, Malik S, Garcia-Bermudez J, Zhu XG, Cantor J, Molina H, Carroll T, Roeder RG, Abdel-Wahab O, Allis CD, Birsoy K. Cell Metab. 2020 Apr 7;31(4):852-861.e6. doi: 10.1016/j.cmet.2020.03.008. PubMed 32268116