mCherry- eMagBF - 5ptaseOCRL
(Plasmid
#162254)
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PurposeBlue-light dependent heterodimerization system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemCherry-CRY2-5-ptaseOCRL (Idevall-Hagren et al., 2012, PNAS)(Plasmid #66836)
- Backbone size w/o insert (bp) 3955
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry- eMagBF - 5ptaseOCRL
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SpeciesSynthetic
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Insert Size (bp)2154
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2020.08.28.272807 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry- eMagBF - 5ptaseOCRL was a gift from Pietro De Camilli (Addgene plasmid # 162254 ; http://n2t.net/addgene:162254 ; RRID:Addgene_162254) -
For your References section:
Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, De Camilli P. Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230. 10.7554/eLife.63230 PubMed 33174843