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Addgene

ER-EGFP-eMagA
(Plasmid #162245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    M18 pCAGGS WPRE electroporation vector (Gray et al., 2016)
  • Backbone size w/o insert (bp) 5897
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CytP450 ER targeting sequencel-EGFP-eMagA
  • Species
    Synthetic
  • Insert Size (bp)
    1377
  • Promoter beta-actin promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site HpaI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.08.28.272807 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ER-EGFP-eMagA was a gift from Pietro De Camilli (Addgene plasmid # 162245 ; http://n2t.net/addgene:162245 ; RRID:Addgene_162245)

  • For your References section:

    Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, De Camilli P. Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230. 10.7554/eLife.63230 PubMed 33174843
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