RAPpGEX
(Plasmid #162241)

• Purpose: Expresses LDL-receptor associated protein (RAP), full length in mammalian cells
• Depositing Lab: Andreas Faissner
• Publication: Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45. doi: 10.1074/jbc.M112.419812. Epub 2013 Apr 24.

Backbone

• Vector backbone: pGEX-4T1 (https://www.addgene.org/vector-database/2876/)
• Backbone manufacturer: GE Healthcare/Amersham Biosciences
• Backbone size w/o insert (bp): 4969
• Total vector size (bp): 6036
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The RAP-GST fusion protein was expressed in Escherichia coli and purified via its GST tag according to standard protocols.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: lrpap1
• Alt name: LDL-receptor-related protein associated protein (RAP)
• Species: M. musculus (mouse)
• Insert Size (bp): 1067
• GenBank ID: 16976
• Entrez Gene: Lrpap1 (a.k.a. AA617339, AI790446, AU042172, C77774, HBP44, RAP)
• Tag / Fusion Protein:
  • GST-tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: GGATCCAGGAAGATGGCGCCTCGAAGAGA
• 3′ sequencing primer: CTCGAGGACATGCTGACAGCTCCCGGT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length mouse RAP was cloned by Eva Hennen into the pGEX4T1 vector that was obtained from GE Healthcare (Amersham Biosciences).