pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)
(Plasmid
#162230)
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PurposeLentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLaG17 GFP nanobody SynNotch tTA
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) was a gift from Wendell Lim (Addgene plasmid # 162230 ; http://n2t.net/addgene:162230 ; RRID:Addgene_162230) -
For your References section:
Engineering synthetic morphogen systems that can program multicellular patterning. Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA. Science. 2020 Oct 16;370(6514):327-331. doi: 10.1126/science.abc0033. 10.1126/science.abc0033 PubMed 33060357