Skip to main content
Addgene

FastLightR-bRaf-mVenus
(Plasmid #162155)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Not sure
  • Backbone size w/o insert (bp) 3934
  • Total vector size (bp) 8008
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FastLightR-bRaf-mVenus
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4074
  • Mutation
    Valine 600 is substituted with Glutamic acid
  • Promoter CMV
  • Tag / Fusion Protein
    • mVenus (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FastLightR-bRaf-mVenus was a gift from Andrei Karginov (Addgene plasmid # 162155 ; http://n2t.net/addgene:162155 ; RRID:Addgene_162155)

  • For your References section:

    Light-Regulated allosteric switch enables temporal and subcellular control of enzyme activity. Shaaya M, Fauser J, Zhurikhina A, Conage-Pough JE, Huyot V, Brennan M, Flower CT, Matsche J, Khan S, Natarajan V, Rehman J, Kota P, White FM, Tsygankov D, Karginov AV. Elife. 2020 Sep 23;9. pii: 60647. doi: 10.7554/eLife.60647. 10.7554/eLife.60647 PubMed 32965214
Related items:
Commonly requested with: