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LightR-bRaf-mVenus
(Plasmid #162154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone size w/o insert (bp) 3934
  • Total vector size (bp) 8008
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LightR-bRaf-mVenus
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4074
  • Mutation
    Valine 600 is substituted with Glutamic acid
  • Promoter CMV
  • Tag / Fusion Protein
    • mVenus (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LightR-bRaf-mVenus was a gift from Andrei Karginov (Addgene plasmid # 162154 ; http://n2t.net/addgene:162154 ; RRID:Addgene_162154)

  • For your References section:

    Light-Regulated allosteric switch enables temporal and subcellular control of enzyme activity. Shaaya M, Fauser J, Zhurikhina A, Conage-Pough JE, Huyot V, Brennan M, Flower CT, Matsche J, Khan S, Natarajan V, Rehman J, Kota P, White FM, Tsygankov D, Karginov AV. Elife. 2020 Sep 23;9. pii: 60647. doi: 10.7554/eLife.60647. 10.7554/eLife.60647 PubMed 32965214
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